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Research Assistant II - Laboratory of Dan Hollern - La Jolla California
Company: Salk Institute for Biological Studies Location: La Jolla, California
Posted On: 04/25/2024
DescriptionSUMMARY: The Research Assistant II position is responsible for, with limited instruction for routine work, performing various cell and molecular biology and biochemistry techniques; library prep, mouse handling and colony maintenance, minor mouse surgeries, mouse breeding and weaning, genotyping, flow cytometry and cell sorting, ELISA, single-cell RNA expression analysis in support of the Principal Investigator (PI) and lab research goals. Range of duties include, but is not limited to, data analysis and data base management, maintenance of tissue, DNA, and other experimental samples, assisting with general laboratory maintenance, including equipment maintenance, and ordering of supplies, and training of new personnel. Duties also include establishing and performing research and analysis on projects that involve the study of human and murine B cells. May provide task instruction, training, and guidance to lower-level personnel. May work on independent projects in addition to supporting postdoctoral researchers in the lab. Carries out and analyzes experimental results with limited instruction. DUTIES AND RESPONSIBILITIES: - Library prep for various omics platforms.
- Plasmid assembly and protein purification, and cloning.
- Handling of tumor specimens in human and murine models.
- Perform various techniques in molecular biology such as: RNA extraction and analysis.
- Maintenance of data sets, lab supplies, and general lab operations.
- Perform tumor studies in mice which include, but are not limited to, xenograft of tumor cell lines and injection of oncogenic lentivirus
- Manage and/or perform maintenance of mouse colony including monitoring and measuring of tumors
- Assist with cell maintenance.
- Prepare standard media and solutions.
- Perform weekly inventory, supply stocking, and cleaning.
- Assist in Hazardous, Biohazardous, and Radioisotope waste disposal and maintain safety records
- Performs other related duties as assigned
SUPERVISORY RESPONSIBILITIES: - This job has no supervisory responsibilities.
QUALIFICATIONS: - Minimum of 2 years of experience in a lab either as technician or assistant.
- Bachelor's Degree (BA) from four-year college or university in Biological Sciences or other scientific related subject matter
- Computer skills required: Inventory Software; Microsoft Office; Project Management Software
COMPETENCIES: - Adaptability - Adapts to changes in the work environment; Manages competing demands; Changes approach or method to best fit the situation; Able to deal with frequent change, delays, or unexpected events.
- Analytical - Synthesizes complex or diverse information; Collects and researches data; Uses intuition and experience to complement data; Designs work flows and procedures.
- Attendance/Punctuality - Is consistently at work and on time; Ensures work responsibilities are covered when absent; Arrives at meetings and appointments on time.
- Customer Service - Manages difficult or emotional customer situations; Responds promptly to customer needs; Solicits customer feedback to improve service; Responds to requests for service and assistance; Meets commitments.
- Dependability - Follows instructions, responds to management direction; Takes responsibility for own actions; Keeps commitments; Commits to long hours of work when necessary to reach goals; Completes tasks on time or notifies appropriate person with an alternate plan.
- Initiative - Volunteers readily; Undertakes self-development activities; Seeks increased responsibilities; Takes independent actions and calculated risks; Looks for and takes advantage of opportunities; Asks for and offers help when needed.
- Interpersonal Skills - Focuses on solving conflict, not blaming; Maintains confidentiality; Listens to others without interrupting; Keeps emotions under control; Remains open to others' ideas and tries new things.
- Judgement - Displays willingness to make decisions; Exhibits sound and accurate judgment; Supports and explains reasoning for decisions; Includes appropriate people in decision-making process; Makes timely decisions.
- Motivation - Sets and achieves challenging goals; Demonstrates persistence and overcomes obstacles; Measures self against standard of excellence; Takes calculated risks to accomplish goals.
- Oral Communication - Speaks clearly and persuasively in positive or negative situations; Listens and gets clarification; Responds well to questions; Demonstrates group presentation skills; Participates in meetings.
- Organizational Support - Follows policies and procedures; Completes administrative tasks correctly and on time; Supports organization's goals and values; Benefits organization through outside activities; Supports affirmative action and respects diversity.
- Planning/Organizing - Prioritizes and plans work activities; Uses time efficiently; Plans for additional resources; Sets goals and objectives; Organizes or schedules other people and their tasks; Develops realistic action plans.
- Problem Solving - Identifies and resolves problems in a timely manner; Gathers and analyzes information skillfully; Develops alternative solutions; Works well in group problem solving situations; Uses reason even when dealing with emotional topics.
- Professionalism - Approaches others in a tactful manner; Reacts well under pressure; Treats others with respect and consideration regardless of their status or position; Accepts responsibility for own actions; Follows through on commitments.
- Teamwork - Balances team and individual responsibilities; Exhibits objectivity and openness to others' views; Gives and welcomes feedback; Contributes to building a positive team spirit; Puts success of team above own interests; Able to build morale and group commitments to goals and objectives; Supports everyone's efforts to succeed.
- Technical Skills - Assesses own strengths and weaknesses; Pursues training and development opportunities; Strives to continuously build knowledge and skills; Shares expertise with others.
- Written Communication - Writes clearly and informatively; Edits work for spelling and grammar; Varies writing style to meet needs; Presents numerical data effectively; Able to read and interpret written information.
- Quality - Demonstrates accuracy and thoroughness; Looks for ways to improve and promote quality; Applies feedback to improve performance; Monitors own work to ensure quality.
- Safety and Security - Observes safety and security procedures; Determines appropriate action beyond guidelines; Reports potentially unsafe conditions; Uses equipment and materials properly.
- Ethics - Treats people with respect; Keeps commitments; Inspires the trust of others; Works with integrity and ethically; Upholds organizational values.
- Diversity - Demonstrates knowledge of EEO policy; Shows respect and sensitivity for cultural differences; Educates others on the value of diversity; Promotes a harassment-free environment; Builds a diverse workforce.
The expected pay range for this position is $21.15-$25.00 per hour. Salk Institute provides pay ranges representing its good faith estimate of what the institute reasonably expects to pay for a position. The pay offered to a selected candidate will be determined based on factors such as (but not limited to) the scope and responsibilities of the position, the qualifications of the selected candidate, department budget availability, internal equity, geographic location, and external market pay for comparable jobs. PHYSICAL DEMANDS AND WORK ENVIRONMENT: - Frequently required to stand
- Frequently required to walk
- Frequently required to sit
- Frequently required to utilize hand and finger dexterity
- Frequently required to climb, balance, bend, stoop, kneel or crawl
- Frequently required to talk or hear
- Frequently required to taste or smell
- Frequently exposure to wet and/or humid conditions (non-weather)
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